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Ies were established from biopsies of metastatic melanoma of cutaneous origin as previously described [9] under the UCLA IRB approval #02-08-067 following the Declaration of Helsinki. SKMEL28, Wn1366 and SBCL2 were obtained from the American Type Culture Collection (ATCC, Rockville, MD). The uveal melanoma cell lines of the Mel20 series were established from fine needle aspirates of primary uveal
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Ies were established from biopsies of metastatic melanoma of cutaneous origin as previously described [9] under the UCLA IRB approval #02-08-067 following the Declaration of Helsinki. SKMEL28, Wn1366 and SBCL2 were obtained from the American Type Culture Collection (ATCC, Rockville, MD). The uveal melanoma cell lines of the Mel20 series were established from fine needle aspirates of primary uveal
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Immediately extracted from the trace elements and lipids by the use of solid phase extraction CHROMABOND column NH2 phase Cat. No. 730031. The sample was then injected directly into an AQUA column 150 54.6 mm 5 C18, purchased from Phenomenex, USA under the following conditions: mobile phase 97/3 20 Mm potassium phosphate, pH 3.0/ methanol, flow rate 1.5 ml/min, UV 270 nm. norepinephrine, dopamine
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G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was f
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Cquire the disease the first time (primary infections) are often asymptomatic and will generate immunity to homologous strains of the virus; however, ninety percent of DHF/ DSS cases come from a second exposure (secondary infection) to a heterologus strain of dengue[5]. Patients with a secondary heterotypic infection are at least 40-80 times more likely to develop DHF/DSS as patients with a primar
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