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E measured by ELISA technique (DRG Diagnostics, Marburg, Germany). We calculated the measurement of free testosterone from measured total testosterone and SHBG. These measurements were obtained between 07.00 am and 11.00 am. There is general agreement that total testosterone levels above 12 nmol/L (346 ng/dL) or free testosterone levels above 250 pmol/L (72 pg/mL) do not require testosterone subst
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